icecold pbs (Thermo Fisher)

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Thermo Fisher icecold pbs
Icecold Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/icecold+pbs/RIPA+buffer/pm42155641-94-25-34
Average 99 stars, based on 1 article reviews

icecold pbs - by Bioz Stars, 2026-09

99/100 stars

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Lysis:

Article Title: Alpha-ketoglutarate mitigates insulin resistance and metabolic inflexibility in a mouse model of Ataxia-Telangiectasia.
Article Snippet: RIP was performed using the Magna RIPTM RNA-binding protein immunoprecipitation kit (Millipore) following strictly the manufacturer’s guidelines. .. Human patient fibroblasts were treated with icecold PBS and PierceTM IP lysis buffer (ThermoFisher) supplemented with RNase inhibitors and a protease inhibitor cocktail. ..

Article Title: A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis.
Article Snippet: RIP was performed using the BersinBioTM RIP Kit (BersinBio) according to the manufacturer’s instructions. .. Briefly, 1 × 107 cells were washed with icecold PBS and lysed in RIP Lysis Buffer supplemented with RNase inhibitor (1:100, Thermo Fisher) and protease inhibitor cocktail (Roche) for 10min Communications Biology | (2025) 8:1333 12 on ice. .. After centrifugation (12,000 × g, 10min, 4 °C), supernatants were pre-clearedwith ProteinA/GMagnetic Beads for 1 hr to reduce nonspecific binding.

Article Title: White spot syndrome virus immediate-early protein (wsv100) antagonizes the NF-κB pathway to inhibit innate immune response in shrimp.
Article Snippet: .. In brief, 48 h after transfection, Drosophila S2 cells were harvested and wash with icecold PBS three times and then lysed in IP lysis buffer (Thermo Scientific) with a protease and phosphatase inhibitor cocktail (Merck). .. The supernatants (400 μl) were incubated with 30 μl anti-HA magnetic beads (Thermo Scientifi) or anti-GFP magnetic beads (Smart-Lifesciences) at 4 °C for 4 h. Then the magnetic beads were washed with PBS for five time and subjected to SDS-PAGE assay.

Protease Inhibitor:

Incubation:

Article Title: Selective Inhibition of the ABCG2 Transporter by Primaquine Derivatives Reverses the Multidrug Resistance of Tumor Cells.
Article Snippet: First, the cells were incubated with the tested substances (Ko143 at a final concentration of 1 μM or primaquine derivatives at a final concentration of 1, 5, and 10 μM) and 0.5 μg of unlabeled primary monoclonal antibody 5D3 (IgG2bÎ, Millipore, Burlington, MA, USA, catalog number MAB4155) per sample in a blocking solution containing PBS with 5.5 mM glucose and 1% bovine serum albumin (BSA). .. After incubation at 37 ◦C for 45 min, the cells were washed twice with icecold PBS and incubated with 0.25 μg goat anti-mouse Alexa Fluor 488 secondary antibody (IgG, Thermo Fisher Scientific, Waltham, MA, USA) per sample in a blocking solution containing 0.5% BSA. .. Cells were incubated for 30 min at 37 ◦C, then washed with icecold PBS and analyzed by flow cytometry (BD FACSCalibur, Becton Dickinson, NJ, USA) with excitation at 488 nm and fluorescence collected through a 530 nm pass filter.

Blocking Assay:

Staining:

Article Title: P-Rex1 limits glucose clearance and suppresses hepatocyte glucose uptake and mitochondrial metabolism independently of its Rac-GEF activity.
Article Snippet: .. Icecold PBS was added, and the cells were sedimented at 300 × g for 5 min at 4 ◦ C, stained with cell-permeable MitoTracker Green (MTG, Invitrogen, M7514, 100 nM) and tetramethyl-rhodamine ethyl ester (TMRE, Abcam, ab113852, 200 nM) dyes for 30 min on ice in dark, and washed three times with ice-cold PBS. ..

Transfection:

Light Microscopy:

Article Title: Development of Piperine/HPβCD-Loaded PVA-Coated iron oxide nanoparticles in in situ gel for enhanced retinal delivery and Anti-VEGF activity.
Article Snippet: Piperine (PIP) is a potential therapeutic agent for retinal diseases; however, its poor aqueous solubility limits its ocular bioavailability.. To overcome this limitation, a novel nanocarrier system was fabricated through the adsorption of PIP/hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complexes onto the surface of polymerstabilized iron oxide nanoparticles (IONs), which were then incorporated into an in situ gelling formulation.. Three hydrophilic polymers, i.e., polyethylene glycol, poloxamer 407, and polyvinyl alcohol (PVA) were used as surface coatings.

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